Transposition pattern of a modified Ds element in tomato.

C M Rommens; T R Munyikwa; B Overduin; H J Nijkamp; J Hille

doi:10.1007/bf00023607

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Transposition pattern of a modified Ds element in tomato.

C M Rommens·T R Munyikwa·B Overduin·H J Nijkamp·J Hille

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Abstract

Several aspects of transposition of an in vitro modified Ds element are described. This Ds element, designated Ds-r, is equipped with bacterial plasmid sequences and can, therefore, be rescued from the plant genome. Our results indicate that the Ds-r element has a 'late' timing of transposition from T-DNAs. This feature of the element might be advantageous for tagging experiments because it leads to independently transposed germinally transmitted elements. Furthermore, it is shown that Ds-r transposition generates clusters of insertions, indicating that 'genes to be tagged' should be located in genomic regions covered by insertions.

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Base Sequence Blotting, Southern DNA DNA Transposable Elements DNA, Bacterial Molecular Sequence Data Plants Polymorphism, Restriction Fragment Length Journal Article

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C M Rommens, T R Munyikwa, B Overduin, H J Nijkamp, J Hille. 1993. Transposition pattern of a modified Ds element in tomato.. https://doi.org/10.1007/bf00023607

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