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PubMed · 8107872

DNA recognition by beta-sheets in the Arc repressor-operator crystal structure.

B E Raumann·M A Rould·C O Pabo·R T Sauer
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Abstract

Transcription of the ant gene during lytic growth of bacteriophage P22 (ref. 1) is regulated by the cooperative binding of two Arc repressor dimers to a 21-base-pair operator site. Here we report the co-crystal structure of this Arc tetramer-operator complex at 2.6 A resolution. As expected from genetic and structural studies and from the co-crystal structure of the homologous Escherichia coli MetJ repressor, each Arc dimer uses an antiparallel beta-sheet to recognize bases in the major groove. However, the Arc and MetJ complexes differ in several important ways: the beta-sheet-DNA interactions of Arc are far less symmetrical; DNA binding by Arc is accompanied by important conformational changes in the beta-sheet; and Arc uses a different part of its protein surface for dimer-dimer interactions.

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Base SequenceCrystallography, X-RayDNAModels, MolecularMolecular Sequence DataOperator Regions, GeneticProtein Structure, SecondaryRepressor ProteinsViral ProteinsViral Regulatory and Accessory ProteinsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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B E Raumann, M A Rould, C O Pabo, R T Sauer. 1994-02-24. DNA recognition by beta-sheets in the Arc repressor-operator crystal structure.. https://doi.org/10.1038/367754a0

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