[Anticancer agents and apoptosis].

M Fukuda; N Saijo

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PubMed · 8109988

[Anticancer agents and apoptosis].

M Fukuda·N Saijo

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Abstract

We reviewed recent reports on apoptosis and summarized the presentations at the Shirafu Cancer Symposium, 1993. The study of programmed cell death, apoptosis, has become one of the mainstream in cell biology, particularly in immunology, developmental biology and oncology. To determine whether the apoptotic cell death induced by anti-cancer agents could be inhibited by bcl-2, we established a bcl-2-transfected human small cell lung cancer cell line, SBC-3/Bcl-2. SBC-3/Bcl-2 showed higher resistance to ADM, CPT-11 and MMC compared with the parental line SBC-3. Agarose gel electrophoresis showed typical DNA fragmentation of SBC-3 following treatment with CPT-11 or MMC. In contrast, the same concentration of the drugs did not induce DNA fragmentation in SBC-3/Bcl-2. However, there was no difference in sensitivity to CDDP, VP-16, ACNU, MTX and Taxol between SBC-3 and SBC-3/Bcl-2 (Ohmori, T. et al. Biochem. Biophys. Res. Commun. 1993). These studies indicate that bcl-2 can modulate the cytotoxicity of some anti-cancer agents by inhibiting the process of apoptosis. We speculate that some apoptotic pathways are bcl-2-sensitive and others bcl-2-independent.

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Animals Antineoplastic Agents Apoptosis Carcinoma, Small Cell Drug Resistance Humans Lung Neoplasms Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Rats Transfection Tumor Cells, Cultured English Abstract Journal Article

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M Fukuda, N Saijo. 1994. [Anticancer agents and apoptosis].. https://pubmed.ncbi.nlm.nih.gov/8109988/

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