A liquid, colorimetric presence-absence coliphage detection method.

M M Ijzerman; J O Falkinham; C Hagedorn

doi:10.1016/0166-0934(93)90106-2

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A liquid, colorimetric presence-absence coliphage detection method.

M M Ijzerman·J O Falkinham·C Hagedorn

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Abstract

A liquid, colorimetric presence-absence coliphage detection method based on the induction of beta-galactosidase by Escherichia coli is described. The release of beta-galactosidase in the medium due to lytic cell infections by coliphages permits the hydrolysis of a yellow chromogenic substrate that develops into a distinct red coliphage positive sample, while a coliphage negative sample remains yellow. This method has proven to be rapid, simpler to perform than an agar medium assay, easy to read and interpret, inexpensive, and highly sensitive.

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Chlorophenols Coliphages Colorimetry Enzyme Induction Escherichia coli Evaluation Studies as Topic Galactosides Isopropyl Thiogalactoside Sensitivity and Specificity Sewage Virology Water Microbiology Water Supply beta-Galactosidase Comparative Study Journal Article Research Support, Non-U.S. Gov't

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M M Ijzerman, J O Falkinham, C Hagedorn. 1993-12-15. A liquid, colorimetric presence-absence coliphage detection method.. https://doi.org/10.1016/0166-0934(93)90106-2

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