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Nucleosome structural changes due to acetylation.

W R Bauer·J J Hayes·J H White·A P Wolffe
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Abstract

We have measured the helical repeat of acetylated nucleosomes reconstituted with HeLa cell histone octamers and the 5 S RNA gene positioning sequence from Xenopus borealis. The hydroxyl radical footprinting experiments show that the helical repeat of nucleosome-bound DNA is unchanged upon acetylation, and that the extent of DNA-protein contacts remains unaltered. These results, combined with previous determinations of the linking number change in minichromosomes as a function of the extent of acetylation, are analyzed with surface linking theory. We demonstrate that the shape of the nucleosome changes upon acetylation, in such a way as to decrease the number of times the DNA winds around the nucleosome central axis.

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AcetylationAnimalsDNA, RibosomalHeLa CellsHistonesHumansHydroxyl RadicalMathematicsModels, StructuralModels, TheoreticalNucleosomesRNA, Ribosomal, 5SXenopusJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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W R Bauer, J J Hayes, J H White, A P Wolffe. 1994-02-25. Nucleosome structural changes due to acetylation.. https://doi.org/10.1006/jmbi.1994.1180

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