DNA stretching on functionalized gold surfaces.

R M Zimmermann; E C Cox

doi:10.1093/nar/22.3.492

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DNA stretching on functionalized gold surfaces.

R M Zimmermann·E C Cox

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Abstract

We describe a method for anchoring bacteriophage lambda DNA by one end to gold by Au-biotin-streptavidin-biotin-DNA bonds. DNA anchored to a microfabricated Au line could be aligned and stretched in flow and electric fields. The anchor was shown to resist a force of at least 11 pN, a linkage strong enough to allow DNA molecules of chromosome size to be stretched and aligned.

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Bacteriophage lambda Base Sequence Biotin DNA, Viral Electromagnetic Fields Gold Microscopy, Electron Molecular Sequence Data Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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R M Zimmermann, E C Cox. 1994-02-11. DNA stretching on functionalized gold surfaces.. https://doi.org/10.1093/nar%2F22.3.492

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