Increased salt concentration reversibly destabilizes p53 quaternary structure and sequence-specific DNA binding.

S Butcher; P Hainaut; J Milner

doi:10.1042/bj2980513

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Increased salt concentration reversibly destabilizes p53 quaternary structure and sequence-specific DNA binding.

S Butcher·P Hainaut·J Milner

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Abstract

Growth suppression by p53 correlates with sequence-specific DNA binding and is determined by tertiary and quaternary protein structures. Exposure to 300 mM NaCl did not affect p53 tertiary structure, but dissociated high-molecular-mass complexes with concomitant loss of specific DNA binding. Both effects were reversible. We conclude that high salt can reversibly destabilize the quaternary structure of p53 that is most efficient for sequence-specific DNA binding.

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Animals Base Sequence Binding Sites Cations DNA Immunosorbent Techniques Mice Molecular Sequence Data Molecular Weight Osmolar Concentration Plasmids Protein Biosynthesis Protein Conformation Rabbits Sodium Chloride Transcription, Genetic Tumor Suppressor Protein p53 Journal Article Research Support, Non-U.S. Gov't

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S Butcher, P Hainaut, J Milner. 1994-03-15. Increased salt concentration reversibly destabilizes p53 quaternary structure and sequence-specific DNA binding.. https://doi.org/10.1042/bj2980513

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