Scanning electron microscopy of synaptonemal complexes.

A L Barlow; G Jenkins; I ap Gwynn

doi:10.1007/bf00710602

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Scanning electron microscopy of synaptonemal complexes.

A L Barlow·G Jenkins·I ap Gwynn

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Abstract

The novel application of scanning electron microscopy to study whole-mount surface-spread synaptonemal complex complements of rye (Secale cereale) and rat (Rattus norvegicus) is described. Scanning electron microscopy is able to resolve the third dimension in such preparations and improve the tracing of the continuity of lateral elements without losing information that could be obtained by conventional transmission electron microscopy. This improvement is likely to benefit detailed studies of chromosome synapsis and karyology, and may provide a means of circumventing technical obstacles inhibiting the use of surface-spreads as substrates for in situ hybridization under the electron microscope.

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Animals Chromosomes In Situ Hybridization Male Meiosis Microscopy, Electron Microscopy, Electron, Scanning Rats Secale Spermatocytes Synaptonemal Complex Journal Article Research Support, Non-U.S. Gov't

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A L Barlow, G Jenkins, I ap Gwynn. 1993. Scanning electron microscopy of synaptonemal complexes.. https://doi.org/10.1007/bf00710602

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