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Transcription factor GATA-1-multiprotein complexes and chicken erythroid development.

C G Penner·J R Davie
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Abstract

The chicken erythrocyte transcription factor, GATA-1, is associated with several non-DNA binding proteins. We show that GATA-1 multiprotein complexes exist in primitive and definitive erythrocytes. These complexes bind to GATA motifs of the rho-globin promoter and histone H5 enhancer with high affinity, and to the chicken beta-globin promoter specialized TATA element and enhancer GATA with low affinity. The low affinity beta-globin TATA element would allow basal transcription factors to displace the GATA-1 multiprotein complex. Further, our results suggest that rho-globin promoter's low affinity Sp1 binding site and reduced levels of Sp1 in definitive cells prevent its expression in these cells.

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AnimalsBase SequenceBinding SitesCell NucleusChickensDNA-Binding ProteinsEnhancer Elements, GeneticErythrocytesErythroid Precursor CellsErythroid-Specific DNA-Binding FactorsErythropoiesisGlobinsHistonesMacromolecular SubstancesMolecular Sequence DataMultiprotein ComplexesOligodeoxyribonucleotidesPromoter Regions, GeneticTranscription FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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C G Penner, J R Davie. 1994-04-11. Transcription factor GATA-1-multiprotein complexes and chicken erythroid development.. https://doi.org/10.1016/0014-5793(94)80515-6

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