Interleukin-1B: a clinically relevant urinary marker.

S M Martins; D J Darlin; P M Lad; P E Zimmern

doi:10.1016/s0022-5347(17)35212-6

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Interleukin-1B: a clinically relevant urinary marker.

S M Martins·D J Darlin·P M Lad·P E Zimmern

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Abstract

Urinary cytokines as markers for the intravesical inflammatory response have become an active area of research. Interleukin-1b, a well studied and early produced cytokine in the immune recognition cascade, was evaluated. After extensive analysis of 56 control and study group urine samples, a simplified and reliable protocol for the preparation of urine before cytokine analysis was devised. The application of an available serum interleukin-1b enzyme-linked immunosorbent assay kit for urinary interleukin-1b analysis was then tested. Finally, a reference value for interleukin-1b in normal human urine was established and urinary interleukin-1b was measured in various bladder diseases. The normal and interstitial cystitis groups showed no interleukin-1b elevation. Significant elevation of urinary interleukin-1b was found in patients with bacterial cystitis compared to the interstitial cystitis and control groups (p < 0.001). Of the patients with bladder tumors 58% showed elevation of urinary interleukin-1b (p < 0.001). Urinary interleukin-1b may be used as a marker to distinguish between bacterial and interstitial cystitis. The absence of urinary interleukin-1b in interstitial cystitis argues against an immunological or autoimmune etiology of the disorder. This study represents an important methodological approach to cytokine subtyping of bladder diseases.

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Adolescent Adult Aged Aged, 80 and over Biomarkers Child Child, Preschool Cystitis Enzyme-Linked Immunosorbent Assay Female Humans Immunoenzyme Techniques Interleukin-1 Male Middle Aged Urinary Bladder Neoplasms Journal Article

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S M Martins, D J Darlin, P M Lad, P E Zimmern. 1994. Interleukin-1B: a clinically relevant urinary marker.. https://doi.org/10.1016/s0022-5347(17)35212-6

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