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PubMed · 8163022

Serpins from wheat grain.

I Rosenkrands·J Hejgaard·S K Rasmussen·S E Bjørn
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Abstract

Wheat serpin genes have been identified by Southern blot hybridization with three distinct barley protein Z probes. Immunoblot analysis with a monoclonal antibody towards barley protein Z confirmed expression of related M(r) approximately 40 kDa proteins in wheat grain. The wheat serpins were extracted under reducing conditions and separated from beta-amylase and other seed proteins by thiophilic adsorption and anion-exchange chromatography. One molecular form possessing chymotrypsin inhibitory activity was isolated in a reactive site cleaved form on a chymotrypsin affinity column. N-terminal amino acid sequences of a CNBr fragment and of the C-terminal peptide from the cleaved inhibitor (M(r) 4574 +/- 4 Da) verified homology with barley protein Z and mammalian serpins. The native inhibitory serpin was demonstrated to form an SDS-stable complex with alpha-chymotrypsin.

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Amino Acid SequenceAnimalsBlotting, SouthernChromatography, GelChromatography, Ion ExchangeElectrophoresis, Polyacrylamide GelHumansMolecular Sequence DataSequence AlignmentSerpinsTriticumJournal ArticleResearch Support, Non-U.S. Gov't

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I Rosenkrands, J Hejgaard, S K Rasmussen, S E Bjørn. 1994-04-18. Serpins from wheat grain.. https://doi.org/10.1016/0014-5793(94)80610-1

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