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PubMed · 8167007

A rapid density gradient technique for separating polymorphonuclear granulocytes.

A Cabanis·B Gressier·S Lebegue·C Brunet·T Dine·M Luyckx·M Cazin·J C Cazin
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Abstract

A gradient separation technique followed by isotonic ammonium chloride haemolysis was compared with two methods for the isolation of polymorphonuclear neutrophils from blood. This technique provided a high yield, excellent purity without lymphocyte and erythrocyte contamination, and made it possible to isolate more than 50 x 10(6) human neutrophils from 15 ml of blood. The polymorphonuclear neutrophils isolated in this way were capable of generating a large amount of reactive oxygen species. This technique for the separation of polymorphonuclear neutrophils is an effective method for in vitro studies.

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Cell SeparationCell SurvivalCentrifugation, Density GradientHemolysisHumansHydrogen PeroxideLeukocyte CountLymphocytesNeutrophilsSuperoxidesComparative StudyJournal Article

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A Cabanis, B Gressier, S Lebegue, C Brunet, T Dine, M Luyckx, M Cazin, J C Cazin. 1994. A rapid density gradient technique for separating polymorphonuclear granulocytes.. https://doi.org/10.1111/j.1699-0463.1994.tb04855.x

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