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PubMed · 8176335

A new method for expressing axonal size: rat optic nerve analysis.

R Duvdevani·V Lavie·L Segel·M Schwartz
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Abstract

Analysis of the shape of the cross sections of adult rat optic nerve axons reveals that the majority of axons do not have a true circular shape. Therefore, determination of axonal size has to utilize methods of approximation. The method presented here utilizes three calculated parameters for expression of axonal size: (i) axonal diameter, as calculated from its area, or (ii) axonal diameter, as calculated from its perimeter, both assuming axonal shape to be a perfect circle and (iii) axonal shape factor, which represents the divergence of the axon from a perfect circular shape. The use of the calculated axonal diameter, with a correction for its shape factor, provides a normalized way of expressing axonal size.

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AnimalsAxonsImage Processing, Computer-AssistedMicroscopy, ElectronOptic NerveRatsRats, Sprague-DawleyJournal Article

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R Duvdevani, V Lavie, L Segel, M Schwartz. 1993. A new method for expressing axonal size: rat optic nerve analysis.. https://pubmed.ncbi.nlm.nih.gov/8176335/

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