[Oligonucleotide directed in vitro mutagenesis].

G Su

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PubMed · 8178511

[Oligonucleotide directed in vitro mutagenesis].

G Su

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Abstract

In this study, a method for oligonucleotide directed in vitro mutagenesis was described. It includes the following steps: clone of the gene to be mutated into the phagemid pGCI; preparation of single strand template; design and synthesis of mutated oligonucleotides; synthesis of double strand DNA. Using this method, a new BamHI site was originated at the N terminal of Stx-B and BglII site at the C terminal of Stx-B. It is ready to fuse the Stx-B gene to LamB. This method is a very useful tool for genetic engineering.

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Base Sequence Escherichia coli Molecular Sequence Data Mutagenesis, Site-Directed Oligonucleotides Plasmids Journal Article

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G Su. 1993. [Oligonucleotide directed in vitro mutagenesis].. https://pubmed.ncbi.nlm.nih.gov/8178511/

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