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PubMed · 8191545

Active, interactive, and inactive steroid receptor mutants.

R B Lanz·M Hug·M Gola·T Tallone·S Wieland·S Rusconi
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Abstract

In transient co-transfection assays, there is extensive cross-interaction between glucocorticoid receptor (GR) domains. For example, mutation of the conserved Ile residue at position 484 (rat GR map) to cysteine allows a net separation of transactivation and DNA binding. We also observed that the ligand binding domain plays a key role in cooperative transactivation. Furthermore, some carboxy-located mutations markedly alter the response of GR to agonists and antagonists. Finally, different reading frames of the CAG repeat that normally produces an amino-located poly-Gln repeat profoundly affect GR transactivation without altering DNA or ligand binding. This trans-dominant negative phenotype, seen when the CAG repeat yields a poly-Ala stretch, may turn out to be an excellent tool for functional analysis of GR in transgenic organisms.

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Amino Acid SequenceBase SequenceBinding SitesDNAEnhancer Elements, GeneticMolecular Sequence DataMutationPromoter Regions, GeneticReceptors, GlucocorticoidRepetitive Sequences, Nucleic AcidTranscriptional ActivationTransfectionZinc FingersJournal ArticleResearch Support, Non-U.S. Gov't

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R B Lanz, M Hug, M Gola, T Tallone, S Wieland, S Rusconi. 1994. Active, interactive, and inactive steroid receptor mutants.. https://doi.org/10.1016/0039-128x(94)90093-0

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