Ethidium bromide intercalation and chromatin structure: a spectropolarimetric analysis.

L Vergani; P Gavazzo; G Mascetti; C Nicolini

doi:10.1021/bi00187a027

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Ethidium bromide intercalation and chromatin structure: a spectropolarimetric analysis.

L Vergani·P Gavazzo·G Mascetti·C Nicolini

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Abstract

Native chromatin has been characterized at different ionic strengths, according to different methods of preparation, by means of circular dichroism and ethidium bromide intercalation. For a more precise interpretation of these results, a series of analogous experiments on phagic DNA, as a function of its circularization, and on mononucleosomes have been performed. Results are discussed here in terms of high order chromatin structure, DNA supercoiling, and topological constraints.

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Animals Bacteriophage lambda Cattle Chromatin Circular Dichroism DNA, Superhelical DNA, Viral Ethidium Nucleic Acid Conformation Protein Conformation Journal Article Research Support, Non-U.S. Gov't

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L Vergani, P Gavazzo, G Mascetti, C Nicolini. 1994-05-31. Ethidium bromide intercalation and chromatin structure: a spectropolarimetric analysis.. https://doi.org/10.1021/bi00187a027

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