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Biologically active recombinant human complement factor H: synthesis and secretion by the baculovirus system.

A K Sharma·M K Pangburn
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Abstract

A complete cDNA clone (4.3 kb) encoding human complement factor H (hCFH; 155 kDa) has been cloned into the pVL1393 baculovirus expression vector and transfected into Spodoptera frugiperda Sf9 insect cells. A biologically active (92 +/- 15%) 140-kDa protein was secreted into the medium with a yield of more than 5 mg/liter. This is the first report of synthesis of biologically active recombinant hCFH in a heterologous system.

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AnimalsBaculoviridaeComplement C3bComplement Factor HGenetic VectorsHumansMothsRecombinant ProteinsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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A K Sharma, M K Pangburn. 1994-06-10. Biologically active recombinant human complement factor H: synthesis and secretion by the baculovirus system.. https://doi.org/10.1016/0378-1119(94)90116-3

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