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Hybridization-based affinity partitioning of nucleic acids using PEG-coupled oligonucleotides.

A Jäschke·J P Fürste·V A Erdmann·D Cech
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Abstract

Polyethylene glycol (PEG)-coupled oligonucleotides are partitioned in an aqueous two-phase system PEG/dextran. The affinity of the oligonucleotide for the PEG-rich phase increases proportionally to the length of the coupled PEG polymer. After hybridization, the PEG-coupled oligonucleotide is able to force a complementary nucleic acid strand into the PEG-rich phase. This property can be used for the sequence-specific isolation of nucleic acids through hybridization-based affinity partitioning. The dependence of the partition coefficient in this system on various parameters is described. The application of this principle to multistage chromatographic separations is demonstrated.

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Base SequenceChromatography, AffinityChromatography, High Pressure LiquidMolecular Sequence DataNucleic Acid HybridizationOligodeoxyribonucleotidesPolyethylene GlycolsSolubilityJournal ArticleResearch Support, Non-U.S. Gov't

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A Jäschke, J P Fürste, V A Erdmann, D Cech. 1994-05-25. Hybridization-based affinity partitioning of nucleic acids using PEG-coupled oligonucleotides.. https://doi.org/10.1093/nar%2F22.10.1880

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