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Ferredoxin biosynthesis in Euglena gracilis.

R S Matson·T Kimura
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Abstract

Analysis of ferredoxin content in cultures of Euglena gracilis grown in the presence of selective antibiotic inhibitors of protein synthesis resulted in the following conclusions: 1. Ferredoxin is synthesized from cytoplasmic (80s-type) ribosomes; cycloheximide, a potent inhibitor of 80s translation completely abolished the synthesis, while the inhibitors of 70s translation chloramphenicol and erythromycin were not effective. In addition, ferredoxin was detected in a streptomycin-bleached mutant that lacks the chloroplast structure and chloroplast DNA. 2. Ferredoxin's transcript is presumably of nuclear origin; rifampicin, an inhibitor of chloroplast DNA-dependent RNA polymerase did not inhibit synthesis, while the streptomycin-bleached mutant continued to synthesize ferredoxin without chloroplast DNA.

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AnimalsBiological Transport, ActiveCell DivisionChloramphenicolChlorophyllCycloheximideCytoplasmErythromycinEuglena gracilisFerredoxinsGlucosephosphate DehydrogenaseIronRibosomesRifampinJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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R S Matson, T Kimura. 1976-08-02. Ferredoxin biosynthesis in Euglena gracilis.. https://doi.org/10.1016/0005-2787(76)90178-7

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