Solid-phase extraction protocol for isolating retinol-d4 and retinol from plasma for parallel processing for epidemiological studies.

S R Dueker; J M Lunetta; A D Jones; A J Clifford

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PubMed · 8222228

Solid-phase extraction protocol for isolating retinol-d4 and retinol from plasma for parallel processing for epidemiological studies.

S R Dueker·J M Lunetta·A D Jones·A J Clifford

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Abstract

Solid-phase extraction permits the parallel processing of samples in large numbers. We have applied this technique to the isolation of retinol isotopomers from plasma of humans participating in a study of vitamin A stable isotope dilution. The isotopomers were analyzed by gas chromatography/mass spectrometry. The extraction involves the separation of retinol from its aqueous matrix with a C18 silica-based sorbent followed by removal of lipid contaminants with an aminopropyl silica-based sorbent. Overall recovery of retinol from plasma was 47.2% +/- 1.8%. Purity of the retinol isolated from plasma is comparable with that obtained with a single HPLC method. This method permits the preparation of 32 samples per day by one analyst. Elimination of the need for HPLC permits sample preparation in the field with a minimum of equipment and technical skill.

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Acetonitriles Adsorption Chromatography, High Pressure Liquid Gas Chromatography-Mass Spectrometry Humans Indicator Dilution Techniques Male Middle Aged Propylamines Silicon Dioxide Vitamin A Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

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S R Dueker, J M Lunetta, A D Jones, A J Clifford. 1993. Solid-phase extraction protocol for isolating retinol-d4 and retinol from plasma for parallel processing for epidemiological studies.. https://pubmed.ncbi.nlm.nih.gov/8222228/

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