Sequencing using pulsed field and image reconstruction.

J Noolandi; R Forsyth; A C Shi

doi:10.1073/pnas.90.21.10101

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Sequencing using pulsed field and image reconstruction.

J Noolandi·R Forsyth·A C Shi

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Abstract

The use of pulsed fields in a standard manual sequencing set-up results in the separation of > 2 kb on a single gel, as compared to 300-400 bases with a dc field. However, visual reading of the sequence from a film exposed to a pulsed-field gel is not possible for more than 800-900 bases under the best conditions. The use of image reconstruction and enhancement techniques allows the reading of the M13mp18 sequence to > 1 kb, and individual bands can be identified at > 2 kb.

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Bacteriophage M13 Base Sequence DNA DNA, Viral Electrophoresis, Polyacrylamide Gel Genetic Techniques Indicators and Reagents Mathematics Molecular Sequence Data Probability Templates, Genetic Journal Article Research Support, Non-U.S. Gov't

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J Noolandi, R Forsyth, A C Shi. 1993-11-01. Sequencing using pulsed field and image reconstruction.. https://doi.org/10.1073/pnas.90.21.10101

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