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PubMed · 8248807

A link between cyclin A expression and adhesion-dependent cell cycle progression.

T M Guadagno·M Ohtsubo·J M Roberts·R K Assoian
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Abstract

Cell adhesion has an essential role in regulating proliferation during the G1 phase of the cell cycle, and loss of this adhesion requirement is a classic feature of oncogenic transformation. The appearance of cyclin A messenger RNA and protein in late G1 was dependent on cell adhesion in both NRK and NIH 3T3 fibroblasts. In contrast, the expression of Cdc2, Cdk2, cyclin D1, and cyclin E was independent of adhesion in both cell lines. Transfection of NRK cells with a cyclin A complementary DNA resulted in adhesion-independent accumulation of cyclin A protein and cyclin A-associated kinase activity. These transfected cells also entered S phase and complete multiple rounds of cell division in the absence of cell adhesion. Thus, cyclin A is a target of the adhesion-dependent signals that control cell proliferation.

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3T3 CellsAnimalsCDC2 Protein KinaseCDC2-CDC28 KinasesCell AdhesionCell CycleCell LineCyclin-Dependent Kinase 2Cyclin-Dependent KinasesCyclinsFibroblastsGene Expression RegulationHumansMiceProtein KinasesProtein Serine-Threonine KinasesRatsTransfectionJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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T M Guadagno, M Ohtsubo, J M Roberts, R K Assoian. 1993-12-03. A link between cyclin A expression and adhesion-dependent cell cycle progression.. https://doi.org/10.1126/science.8248807

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