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PubMed · 8251113

mRNA-ribosome interactions.

S S Dokudovskaya·O A Dontsova·S L Bogdanova·A A Bogdanov·R Brimacombe
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Abstract

Synthetic mRNA analogues were constructed with sequences related to the Cro-protein mRNA from lambda-phage and prepared by T7 transcription. Each mRNA contained several thiouridine (thio-U) residues. The regions upstream from the AUG initiator codon of the mRNA were the same in all the messages, whereas in the downstream part the thio-U residues were placed in selected positions. These positions covered the region from +4 to +16 (A in the initiator AUG codon being defined as +1). After binding to the ribosome in the presence of initiator tRNA the thio-U residues were activated by u.v. irradiation and the resulting sites of cross-linking to 16 S rRNA and ribosomal proteins were analysed. Cross-links to several ribosomal proteins were identified in different types of complex. Changes in the conformation of the small ribosomal subunit in different initiation and elongation complexes are discussed.

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Bacteriophage lambdaBase SequenceCross-Linking ReagentsDNA-Binding ProteinsMolecular Sequence DataRNA, MessengerRNA, Ribosomal, 16SRepressor ProteinsRibosomal ProteinsRibosomesTranscription FactorsViral ProteinsViral Regulatory and Accessory ProteinsJournal Article

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S S Dokudovskaya, O A Dontsova, S L Bogdanova, A A Bogdanov, R Brimacombe. 1993. mRNA-ribosome interactions.. https://pubmed.ncbi.nlm.nih.gov/8251113/

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