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PubMed · 8258638

A flow-through microsampling device applied to an ion-exchange chromatography system.

J Nilsson·P Szecsi·C Schafer-Nielsen
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Abstract

A flow-through microsampling device that can be inserted in the column elution flow line of a chromatographic system has been developed. A sample drop (volume 2.2 nl) is ejected when a voltage pulse is applied to a piezo-electric transducer. The drops can be ejected with up to 70 Hz frequency, giving a sample flow range of 0-9 microliters/min. Samples were collected on a moving agarose surface during elution of freshly prepared human serum. The precipitate pattern obtained after immunoelectrophoresis is similar to what would be obtained in a fused rocket electrophoresis of multiple samples from a fraction collector.

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Blood ProteinsChemistry Techniques, AnalyticalChromatography, Ion ExchangeGelsHumansImmunoelectrophoresisMacromolecular SubstancesMembranesJournal Article

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BibTeXRIS

J Nilsson, P Szecsi, C Schafer-Nielsen. 1993. A flow-through microsampling device applied to an ion-exchange chromatography system.. https://doi.org/10.1016/0165-022x(93)90002-6

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