Oligonucleotide circularization by template-directed chemical ligation.

N G Dolinnaya; M Blumenfeld; I N Merenkova; T S Oretskaya; N F Krynetskaya; M G Ivanovskaya; M Vasseur; Z A Shabarova

doi:10.1093/nar/21.23.5403

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PubMed · 8265356

Oligonucleotide circularization by template-directed chemical ligation.

N G Dolinnaya·M Blumenfeld·I N Merenkova·T S Oretskaya·N F Krynetskaya·M G Ivanovskaya·M Vasseur·Z A Shabarova

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Abstract

An efficient method for producing the covalent closure of oligonucleotides on complementary templates by the action of BrCN was developed. A rational design of linear precursor oligonucleotides was studied, and the effect of factors such as oligonucleotide concentration and oligomer-template length ratio was evaluated. The efficiency of circularization was shown to correlate well with the secondary structure of the precursor oligomer (as predicted by a simple computer analysis), hairpin-like structures bearing free termini clearly favouring the circularization reaction. A novel idea, consisting of the incorporation of non-nucleotide insertions in the precursor oligomer (namely, 1,2-dideoxy-D-ribofuranose residues), may render this method universal and highly effective. An original set of assays was developed to confirm the circular structure of the covalently closed oligonucleotides.

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Base Sequence Cyanogen Bromide DNA, Circular DNA, Single-Stranded Hydrogen Bonding Molecular Sequence Data Nucleic Acid Conformation Oligonucleotides Templates, Genetic Journal Article

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N G Dolinnaya, M Blumenfeld, I N Merenkova, T S Oretskaya, N F Krynetskaya, M G Ivanovskaya, M Vasseur, Z A Shabarova. 1993-11-25. Oligonucleotide circularization by template-directed chemical ligation.. https://doi.org/10.1093/nar%2F21.23.5403

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