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High-sensitivity lens-coupled slow-scan CCD camera for transmission electron microscopy.

G Y Fan·M H Ellisman
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Abstract

A lens-coupled slow-scan CCD camera has been built for transmission electron microscopy (TEM) applications. In this design, a leaded glass window, which is coated with a 20 microns layer of red P20 phosphor, is mounted on the bottom of the microscope. A lens assembly and mirror prism, located outside the microscope vacuum below the leaded glass, relays the image onto a back-thinned 1kx1k charge-coupled device (CCD) detector. This CCD is electronically cooled to below -30 degrees C during operation. It is found that X-ray irradiation, generally found to be annoying in fiber-optically coupled CCD cameras, is completely eliminated by this configuration. The collection efficiency of this system, although not as high as some of the fiber-optically coupled CCD cameras, is high enough to achieve single-electron sensitivity under a high-gain mode.

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Equipment DesignMicroscopy, ElectronPhotographyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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G Y Fan, M H Ellisman. 1993. High-sensitivity lens-coupled slow-scan CCD camera for transmission electron microscopy.. https://doi.org/10.1016/0304-3991(93)90019-t

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