Quantitative enzyme cytochemistry during human macrophage development.

R J Sokol; G Hudson; J M Wales; D J Goldstein; N T James

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PubMed · 8270480

Quantitative enzyme cytochemistry during human macrophage development.

R J Sokol·G Hudson·J M Wales·D J Goldstein·N T James

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Abstract

Integrating microdensitometry was used to study changes in the intracellular activity of 4 enzymes during macrophage development. Suspension cultures of blood monocytes from 19 healthy human subjects were examined at 0, 2, 4 and 6 d. Mononuclear phagocytes were harvested by glass adherence and standard methods were used for cytochemical staining for NADH dehydrogenase, succinate dehydrogenase, acid phosphatase and alpha-naphthyl butyrate esterase. All specimens from all subjects were stained at the same time and staining intensities in individual cells were measured at appropriate wavelengths. A highly significant increase in enzyme activity with culture time was found for all 4 enzymes. These increases in mitochondrial, lysosomal and ectoenzyme activities during development indicate the increasing functional capabilities of the macrophages.

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Acid Phosphatase Adult Carboxylic Ester Hydrolases Cell Differentiation Cells, Cultured Female Humans Macrophages Male Middle Aged Monocytes NADH Dehydrogenase Succinate Dehydrogenase Time Factors Journal Article Research Support, Non-U.S. Gov't

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R J Sokol, G Hudson, J M Wales, D J Goldstein, N T James. 1993. Quantitative enzyme cytochemistry during human macrophage development.. https://pubmed.ncbi.nlm.nih.gov/8270480/

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