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PubMed · 827290

Trypsin-induced phospholipase activity in human platelets.

W C Pickett·R L Jesse·P Cohen
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Abstract

Trypsin mediates a release of arachidonic acid with resultant increase in O2 consumption (a reflection of cyclo-oxygenase activity) by whole human platelets that is similar to thrombin's effect on these cells. The trypsin and thrombin effects can be differentiated in two ways: (1) at saturating concentrations the measured effects of trypsin greatly exceed those of thrombin; (2) EGTA [ethanedioxybis(ethylamine)-NNN'N'-tera-acetate] augments the effect of thrombin but not of trypsin. Thus trypsin and thrombin probably act at different loci in the pathway that induces phospholipase activity in human platelets.

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Arachidonic AcidsBlood PlateletsEgtazic AcidHumansOxygen ConsumptionPhospholipasesPlatelet AggregationThrombinTrypsinJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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W C Pickett, R L Jesse, P Cohen. 1976-11-15. Trypsin-induced phospholipase activity in human platelets.. https://doi.org/10.1042/bj1600405

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