Comparing steady counterflow separation with differential chromatography.

D K Roper; E N Lightfoot

doi:10.1016/0021-9673(93)83060-6

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Comparing steady counterflow separation with differential chromatography.

D K Roper·E N Lightfoot

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Abstract

Separation of closely related solutes by steady solid-fluid counterflow is compared with differential separation in a fixed chromatographic bed. Analogous expressions for exit concentration and mean residence time in the two systems are presented. A counterpart to chromatographic resolution is derived for binary steady counterflow separations. Estimated counterflow savings in product-concentration dilution, solvent volume requirement and solid-phase volume requirement obtained with these expressions relative to comparable chromatographic operations are compared with experimental results from adsorptive, simulated moving beds. Analysis of a size-exclusion protein separation suggests counterflow substantially decreases solvent and resin usage relative to conventional, batch operation.

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Chromatography, Liquid Proteins Solvents Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

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D K Roper, E N Lightfoot. 1993-11-12. Comparing steady counterflow separation with differential chromatography.. https://doi.org/10.1016/0021-9673(93)83060-6

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