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Constitutive interferon expression from retroviral vector.

Z Knezic·G Nikcevic·J Marjanovic·V Glisin·Z Popovic
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Abstract

A genomic fragment with the human beta-interferon gene was cloned into a pL3-4, a defective Moloney murine leukemia virus (M-MuLV) vector. Here we show that clones selected after viral infection of mouse NIH 3T3 cells constitutively produced 128 IU/ml of human beta-interferon. Constitutive synthesis of retroviral RNA was confirmed by dot blot hybridization of RNA isolated from two of the selected clones. Poly(I) x poly(C) and cycloheximide induction resulted in an increased RNA level, but this was not reflected in an increased production of biologically active interferon.

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3T3 CellsAnimalsAntiviral AgentsBase SequenceCloning, MolecularCycloheximideGene Expression Regulation, ViralGenetic VectorsHumansInterferon-betaMiceMolecular Sequence DataMoloney murine leukemia virusPoly I-CPoly URNA, Double-StrandedRNA, MessengerJournal ArticleResearch Support, Non-U.S. Gov't

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Z Knezic, G Nikcevic, J Marjanovic, V Glisin, Z Popovic. 1993. Constitutive interferon expression from retroviral vector.. https://doi.org/10.1016/0166-3542(93)90097-3

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