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Crystallization and a preliminary X-ray diffraction study of macrophage migration inhibitory factor from human lymphocytes.

M Suzuki·E Murata·I Tanaka·J Nishihira·M Sakai
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Abstract

Two crystal forms of the macrophage migration inhibitory factor (MIF) from human lymphocytes have been obtained by the hanging drop method of vapor diffusion from ammonium sulfate solution. A trigonal crystal form belongs to the space group P3(1)21 (P3(2)21), with unit cell dimensions of a = b = 96.4 A and c = 105.5 A. Assuming that the asymmetric unit contains two or three dimers, the Vm value is calculated as 2.9 or 1.9, respectively. A second crystal form is orthorhombic, space group P2(1)2(1)2(1) with unit cell dimensions a = 68.4 A, b = 68.8 A and c = 86.8 A. The Vm value is 2.1 for two dimers in the asymmetric unit. Both crystals diffract to at least 1.9 A and are suitable for X-ray crystallographic structure determination at a high resolution.

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Base SequenceCrystallography, X-RayDNA PrimersHumansLymphocytesMacrophage Migration-Inhibitory FactorsMolecular Sequence DataRecombinant ProteinsJournal ArticleResearch Support, Non-U.S. Gov't

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M Suzuki, E Murata, I Tanaka, J Nishihira, M Sakai. 1994-01-21. Crystallization and a preliminary X-ray diffraction study of macrophage migration inhibitory factor from human lymphocytes.. https://doi.org/10.1006/jmbi.1994.1063

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