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Intracellular pH changes during zona drilling.

H T Depypere·L Leybaert
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Abstract

OBJECTIVE: To investigate whether zona drilling of oocytes, a technique used to locally disrupt the zona pellucida, causes important changes in intracellular pH. DESIGN AND METHODS: In a first set of experiments, intracellular pH was measured during zona drilling of mouse oocytes, using pH-sensitive microelectrodes. In a second set of experiments human oocytes that failed to fertilize were used to measure intracellular pH during drilling. In these cells intracellular pH was measured using microfluorimetry with the pH-sensitive dye 2',7'-bis-carboxyethyl-5(6)-carboxyfluorescein. RESULTS: In mouse oocytes intracellular pH dropped from 7.25 +/- 0.02 (mean +/- SEM) to 7.09 +/- 0.03 during zona drilling, followed by a recovery to pH 7.17 +/- 0.02 after 4 minutes (n = 20). In human oocytes, intracellular pH dropped over 0.36 +/- 0.10 pH units during drilling, followed by a recovery that was complete within 4 minutes (n = 14). CONCLUSION: Zona drilling is associated with a significant cytoplasmic acidification both in mouse and human oocytes. This effect is perhaps related to the high incidence of cytoplasmic degeneration after zona drilling of human oocytes.

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AnimalsFemaleFertilization in VitroFluoresceinsFluorescent DyesHumansHydrogen-Ion ConcentrationMiceMice, Inbred C57BLMice, Inbred CBAMicroelectrodesOocytesZona PellucidaJournal Article

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H T Depypere, L Leybaert. 1994. Intracellular pH changes during zona drilling.. https://doi.org/10.1016/s0015-0282(16)56525-x

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