Sequence analysis and functional studies of interleukin-3 receptor alpha subunit-encoding cDNAs amplified from KG-1 leukemic cells and normal human marrow.

A P Rapoport; J F DiPersio

doi:10.1016/0378-1119(93)90030-7

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Sequence analysis and functional studies of interleukin-3 receptor alpha subunit-encoding cDNAs amplified from KG-1 leukemic cells and normal human marrow.

A P Rapoport·J F DiPersio

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Abstract

Two partial cDNAs encoding the human interleukin-3 receptor alpha subunit (IL-3R alpha) were cloned from KG-1 leukemic cells using the polymerase chain reaction. Sequence analysis of these cDNAs predicted two single-amino-acid (aa) changes as compared with the published TF-1 leukemic cell IL-3R alpha sequence [Kitamura et al., Cell 66 (1991) 1165-1174]. These changes were confirmed by sequence analysis of a second set of independently derived cDNAs. Identical aa changes were found in the IL-3R alpha encoded by cDNAs cloned from normal CD34+ human marrow cells. Ligation of the partial cDNAs derived from KG-1 cells resulted in a full-length functional IL-3R alpha cDNA clone. Deletion of the extracellular 'LSXWS' consensus sequence resulted in complete loss of detectable [125I]IL-3 binding when this mutant receptor was co-expressed in COS-7 cells with the beta subunit of the IL-3 receptor.

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Base Sequence Bone Marrow Bone Marrow Cells Cells, Cultured DNA, Complementary Humans Leukemia Molecular Sequence Data Mutation Polymerase Chain Reaction Receptors, Interleukin-3 Tumor Cells, Cultured Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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A P Rapoport, J F DiPersio. 1993-12-31. Sequence analysis and functional studies of interleukin-3 receptor alpha subunit-encoding cDNAs amplified from KG-1 leukemic cells and normal human marrow.. https://doi.org/10.1016/0378-1119(93)90030-7

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