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PubMed · 8302942

A useful cloning vector for Bacillus subtilis.

B Diderichsen·G B Poulsen·S T Jørgensen
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Abstract

We have constructed plasmid pDN1050, a new small cloning vector for Bacillus subtilis. pDN1050 harbors the origin of replication of Staphylococcus aureus plasmid pUB110 and the chloramphenicol resistance gene of S. aureus plasmid pC194. The plasmid is segregationally and structurally stable. Plasmid pDN1370, a low copy number mutant of pDN1050 was isolated and shown to harbor a mutation in the repA gene of the replication protein.

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Bacillus subtilisBase SequenceCloning, MolecularDNA, BacterialGene DeletionGenetic VectorsMolecular Sequence DataPlasmidsRecombination, GeneticRestriction MappingJournal Article

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B Diderichsen, G B Poulsen, S T Jørgensen. 1993. A useful cloning vector for Bacillus subtilis.. https://doi.org/10.1006/plas.1993.1066

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