An improved MTT assay using the electron-coupling agent menadione.

H Garn; H Krause; V Enzmann; K Drössler

doi:10.1016/0022-1759(94)90062-0

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An improved MTT assay using the electron-coupling agent menadione.

H Garn·H Krause·V Enzmann·K Drössler

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Abstract

A modification of the MTT based tetrazolium colorimetric assay is described. Using the electron-coupling agent menadione formazan formation by murine splenocytes and P-815 cells was significantly increased whereas dye reduction by macrophages was hardly influenced. These observations suggest that it should be possible to improve the tetrazolium based cytotoxicity assays of murine macrophages against cells of the syngeneic tumour cell line P-815.

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Animals Colorimetry Coloring Agents Cytotoxicity Tests, Immunologic Dose-Response Relationship, Drug Macrophages Mice Mice, Inbred CBA Oxidation-Reduction Rats Rats, Wistar Spleen Tetrazolium Salts Thiazoles Tumor Cells, Cultured Vitamin K Journal Article Research Support, Non-U.S. Gov't

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H Garn, H Krause, V Enzmann, K Drössler. 1994-02-10. An improved MTT assay using the electron-coupling agent menadione.. https://doi.org/10.1016/0022-1759(94)90062-0

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