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PubMed · 8312393

Multiple leader sequences for mouse cathepsin B mRNA?

H Rhaissi·D Béchet·M Ferrara
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Abstract

We previously described the gene structure of murine cathepsin B. Our results suggested that the 5'-untranslated region (leader) is interrupted by a large intron. The second exon (exon-2) contains the translation initiation site. To characterize the leader region, a rapid amplification of cDNA ends (RACE) procedure was developed. The PCR products were directly cloned and sequenced. Nucleotide sequence analyses revealed three different 5'-cDNA ends, suggesting the existence of three different leader regions. In addition to the leader (LA) previously characterized, we now describe two other 5'-untranslated regions, LB and LC. Leader LB is located 2.3 kb upstream exon-2, and leader LC corresponds to the 3'-end of the first intron and is thus contiguous to exon-2. Our results suggest for murine cathepsin B gene the presence of multiple promoters, and possibly the expression of multiple mRNAs differing in their leader region.

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3T3 CellsAnimalsBase SequenceCathepsin BCloning, MolecularDNA, ComplementaryExonsIntronsKirsten murine sarcoma virusMiceMolecular Sequence DataPolymerase Chain ReactionPromoter Regions, GeneticRNA, MessengerTranscription, GeneticJournal ArticleResearch Support, Non-U.S. Gov't

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H Rhaissi, D Béchet, M Ferrara. 1993. Multiple leader sequences for mouse cathepsin B mRNA?. https://doi.org/10.1016/0300-9084(93)90046-u

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