[Ultrastructural study of guinea pig optic nerve head using the deep-etch method].

M Furuta; S Tsukahara; J D Lindsey; R N Weinreb

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PubMed · 8317355

[Ultrastructural study of guinea pig optic nerve head using the deep-etch method].

M Furuta·S Tsukahara·J D Lindsey·R N Weinreb

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Abstract

Normal guinea pig optic nerve heads were examined using the quick-freeze, deep-etch, rotary-shadow technique. This method visualized the three-dimensional organization of the cytoskeleton. The axon cytoplasm was composed of 20-25 nm in diameter microtubules (MTs) and 10-12 nm in diameter neurofilaments (NFs) with numerous cross-linkers. These cross-linkers spanned between adjacent NFs, adjacent MTs, and between NFs and MTs. The membranous organelles such as mitochondria, axoplasmic reticulum, or tubulo-vesicular structures also were attached to the NFs and MTs with the cross-linkers. Abundant intermediate filaments of 10-12 nm diameter within glial cell components segregated axon bundles from laminar collagen fibers. The laminar fibers contained interstitial collagens that were 30-60 nm in diameter with characteristic striae and sidearms, and 5-10 nm wide mesh-like structures connecting the collagen fibril bundles to a surrounding basal lamina.

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Animals Freeze Etching Guinea Pigs Optic Disk English Abstract Journal Article Research Support, Non-U.S. Gov't

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M Furuta, S Tsukahara, J D Lindsey, R N Weinreb. 1993. [Ultrastructural study of guinea pig optic nerve head using the deep-etch method].. https://pubmed.ncbi.nlm.nih.gov/8317355/

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