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PubMed · 833013

Bone alkaline phosphatase kinetics studied by a new method.

H Troyer·O T Fisher·T H Rosenquist
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Abstract

Studies of enzyme kinetics by histochemical methods has been seriously impaired by available methods. This study combines two newer methods, i.e., sectioning undecalcified bone from glycol methacrylate and the use of atomic absorpton spectrophotometry for photographic densitometry. The results indicate that the substrate concentration for zero order kinetics of the alkaline phosphatase reaction in bone is 0.5 mg/ml of substrate; and the Km of the same reaction is estimated to be 0.25 mg/ml or 0.56 mM of substrate.

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Alkaline PhosphataseAnimalsBone and BonesDensitometryGuinea PigsHistocytochemistryKineticsMicrotomySpectrophotometry, AtomicJournal Article

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H Troyer, O T Fisher, T H Rosenquist. 1977-01-24. Bone alkaline phosphatase kinetics studied by a new method.. https://doi.org/10.1007/bf00491072

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