An improved method for perforated patch recordings using nystatin-fluorescein mixture.

H Yawo; N Chuhma

doi:10.2170/jjphysiol.43.267

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An improved method for perforated patch recordings using nystatin-fluorescein mixture.

H Yawo·N Chuhma

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Abstract

This paper describes an improved method for solubilizing nystatin in an aqueous solution without the aid of organic solvents. The patch pipette was filled with a solution of nystatin and fluorescein sodium mixed in a molar ratio of 1:10. The success rate of perforated patch recordings was substantially improved, and the access resistance of 20-40 M omega was readily achieved.

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Action Potentials Animals Chick Embryo Electrophysiology Evaluation Studies as Topic Fluorescein Fluoresceins Membrane Potentials Neurons Nystatin Solutions Journal Article Research Support, Non-U.S. Gov't

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H Yawo, N Chuhma. 1993. An improved method for perforated patch recordings using nystatin-fluorescein mixture.. https://doi.org/10.2170/jjphysiol.43.267

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