Human kappa chain expression in a lambda phage vector: methods of isolating amplified cDNA affects cloning efficiency.

K Lazaridis; H Dang; N Talal

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PubMed · 8357956

Human kappa chain expression in a lambda phage vector: methods of isolating amplified cDNA affects cloning efficiency.

K Lazaridis·H Dang·N Talal

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Abstract

Three common methods of isolating amplified DNA were evaluated for their effects on cloning efficiency in a phage vector designed to express human kappa chain. The "glass milk" technique gave higher cloning efficiency and protein expression than phenol-chloroform extraction or microfiltration. This shows that the quality of amplified cDNA should be considered when studying the human antibody repertoire using this vector system.

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Bacteriophage lambda Base Sequence Cloning, Molecular DNA Genes, Immunoglobulin Genetic Vectors Humans Immunoglobulin kappa-Chains Molecular Sequence Data Polymerase Chain Reaction Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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K Lazaridis, H Dang, N Talal. 1993. Human kappa chain expression in a lambda phage vector: methods of isolating amplified cDNA affects cloning efficiency.. https://pubmed.ncbi.nlm.nih.gov/8357956/

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