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Method for sequencing synthetic oligodeoxynucleotide phosphorothioates.

J Tang·A M Roskey·S Agrawal
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Abstract

Sequencing of oligodeoxynucleotide phosphorothioate by a modified Sanger method of sequencing is described. The procedure involves ligation of synthetic oligodeoxynucleotide phosphorothioate to an oligodeoxynucleotide, referred to here as "helper oligonucleotide." The helper oligonucleotide has a region which is complementary to T7 primer. By using DNA polymerase and nucleoside triphosphate mixture, 5'-labeled T7 primer is extended onto ligated oligodeoxynucleotide phosphorothioate, which is then analyzed on gel electrophoresis.

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Base SequenceDNA-Directed DNA PolymeraseElectrophoresis, Polyacrylamide GelEvaluation Studies as TopicMolecular Sequence DataOligodeoxyribonucleotidesOligonucleotide ProbesSequence Analysis, DNATaq PolymeraseJournal Article

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BibTeXRIS

J Tang, A M Roskey, S Agrawal. 1993. Method for sequencing synthetic oligodeoxynucleotide phosphorothioates.. https://doi.org/10.1006/abio.1993.1302

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