A permanent cell viability assay using alcian blue.

J T Thornthwaite; R C Leif

doi:10.3109/10520297809111466

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A permanent cell viability assay using alcian blue.

J T Thornthwaite·R C Leif

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Abstract

The alcian blue dye exclusion method for glutaraldehyde-fixed cells has been utilized with "centrifugal cytology" to prepare permanent records of the viability of individual cells present in suspensions. The viability of spleen cell suspensions separated by linear bovine serum albumin density gradient centrifugation has been measured with this method. Combined light and scanning electron microscopy of nonviable and viable cells demonstrated membrane alterations in alcian blue-stained nonviable cells, while viable cells were spherical and displayed uniform surface features.

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Alcian Blue Animals Cell Membrane Cell Survival Cells, Cultured Centrifugation, Density Gradient Hot Temperature Indoles Mice Spleen Staining and Labeling Trypan Blue Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

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J T Thornthwaite, R C Leif. 1978. A permanent cell viability assay using alcian blue.. https://doi.org/10.3109/10520297809111466

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