Modeling the Ames Salmonella/microsome assay.

D Krewski; B G Leroux; S R Bleuer; L H Broekhoven

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PubMed · 8369384

Modeling the Ames Salmonella/microsome assay.

D Krewski·B G Leroux·S R Bleuer·L H Broekhoven

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Abstract

Of the many short-term tests for mutagenicity that have been proposed in recent years, the Ames Salmonella/microsome assay is the single most widely used and most thoroughly validated in vitro test system. This assay uses cells cultured in a soft agar containing a trace amount of histidine to allow growth of auxotrophic bacteria, and is designed to detect reverse mutations from auxotrophic cells to histidine-independent prototrophic cells. In this paper, statistical models that have been proposed for the analysis of Ames test data are reviewed, including those of a mechanistic and empirical nature. An extension to the class of biologically based models derived by Margolin, Kaplan, and Zeiger (1981, Proceedings of the National Academy of Sciences 78, 3779-3783) is proposed by allowing for diffusion of histidine within the plate agar. Quasi-likelihood methods for estimating the model parameters are presented, and applied to 1,120 data sets from a recent collaborative trial sponsored by the International Programme on Chemical Safety.

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Animals Biotransformation Dose-Response Relationship, Drug Mathematics Microsomes, Liver Models, Statistical Mutagenicity Tests Mutagens Salmonella typhimurium Journal Article Research Support, Non-U.S. Gov't

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D Krewski, B G Leroux, S R Bleuer, L H Broekhoven. 1993. Modeling the Ames Salmonella/microsome assay.. https://pubmed.ncbi.nlm.nih.gov/8369384/

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