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PubMed · 8371973

A simple and efficient method for site-directed mutagenesis with double-stranded plasmid DNA.

D Lai·X Zhu·S Pestka
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Abstract

A general, simple and efficient method for preparing site-specific mutations in double-stranded plasmid DNA without the need for special plasmids, bacterial strains or reagents is described. Only one synthetic oligonucleotide for each mutation is required, subcloning is unnecessary and a high efficiency of mutation (58-97%) was obtained. If two synthetic oligonucleotide primers are used, two separate mutations can be simultaneously created in a single reaction tube.

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Base SequenceDNA, BacterialEscherichia coliGenetic TechniquesMolecular Sequence DataMutagenesis, Site-DirectedPlasmidsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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D Lai, X Zhu, S Pestka. 1993-08-25. A simple and efficient method for site-directed mutagenesis with double-stranded plasmid DNA.. https://doi.org/10.1093/nar%2F21.17.3977

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