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PubMed · 8377830

Structure and function of endoglucanase V.

G J Davies·G G Dodson·R E Hubbard·S P Tolley·Z Dauter·K S Wilson·C Hjort·J M Mikkelsen·G Rasmussen·M Schülein
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Abstract

Cellulose is the major polysaccharide component of plant cell walls and is the most abundant organic compound on the planet. A number of bacterial and fungal organisms can use cellulose as a food source, possessing cellulases (cellobiohydrolases and endoglucanases) that can catalyse the hydrolysis of the beta-(1,4) glycosidic bonds. They can be classified into seven distinct families. The three-dimensional structures of members of two of these families are known. Here we report the structure of a third cellulase, endoglucanase V, whose sequence is not represented in any of the above families. The enzyme is structurally distinct from the previously determined cellulases but is similar to a recently characterized plant defence protein. The active site region resembles that of lysozyme, despite the lack of structural similarity between these two enzymes.

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Binding SitesCellulaseComputer GraphicsMitosporic FungiMuramidaseProtein ConformationX-Ray DiffractionJournal ArticleResearch Support, Non-U.S. Gov't

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G J Davies, G G Dodson, R E Hubbard, S P Tolley, Z Dauter, K S Wilson, C Hjort, J M Mikkelsen, G Rasmussen, M Schülein. 1993-09-23. Structure and function of endoglucanase V.. https://doi.org/10.1038/365362a0

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