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Aligning histological serial sections for three-dimensional reconstruction using an excimer laser beam.

G E Clarke·P W Hamilton·W A Montgomery
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Abstract

Accurate alignment of histological sections has always been a fundamental problem in the three-dimensional reconstruction of tissue structures from serial sections. An excimer laser was used to create small holes in paraffin processed colorectal tissue blocks. The laser holes were 200-230 microns in diameter and penetrated the tissue approximately 0.5 mm. No damage was caused to the surrounding tissue. Using a VIDAS image analyser, these microscopic holes could be used as artificial reference points (ARPs) for the accurate alignment of consecutive serial sections and three-dimensional reconstruction of colorectal glandular tissue.

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ColonHistological TechniquesHumansImage Processing, Computer-AssistedLasersRectumJournal Article

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G E Clarke, P W Hamilton, W A Montgomery. 1993. Aligning histological serial sections for three-dimensional reconstruction using an excimer laser beam.. https://doi.org/10.1016/s0344-0338(11)80367-5

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