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PubMed · 8378352

A model for tumor suppression using H-1 parvovirus.

A Telerman·M Tuynder·T Dupressoir·B Robaye·F Sigaux·E Shaulian·M Oren·J Rommelaere·R Amson
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Abstract

A model system is proposed to investigate, at the molecular level, the pathways of tumor suppression. As a tool for the selection of cells with a suppressed phenotype, we used the H-1 parvovirus that preferentially kills various neoplastic cells. From the human K562 leukemia cells, we isolated a clone, KS, that is resistant to the cytopathic effect of the H-1 virus and displays a suppressed malignant phenotype. The suppressed malignancy and the cellular resistance to H-1 killing appear to depend on the activity of wild-type p53. Whereas the KS cells express wild-type p53, the protein is undetectable in the parental K562 cells. Experiments with p53 mutants suggest that wild-type p53, in its functionally intact state, contributes to the resistance against the cytopathic effect of H-1 parvovirus.

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Antigens, CDBase SequenceBlotting, WesternCell SurvivalClone CellsDNA, ViralElectrophoresis, Gel, Two-DimensionalGenes, Tumor SuppressorGenes, p53HumansLeukemia, Myelogenous, Chronic, BCR-ABL PositiveMethionineMolecular Sequence DataMolecular WeightNeoplasm ProteinsOligodeoxyribonucleotidesParvoviridaePhenotypePolymerase Chain ReactionSuppression, GeneticTumor Cells, CulturedTumor Suppressor Protein p53Journal ArticleResearch Support, Non-U.S. Gov't

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A Telerman, M Tuynder, T Dupressoir, B Robaye, F Sigaux, E Shaulian, M Oren, J Rommelaere, R Amson. 1993-09-15. A model for tumor suppression using H-1 parvovirus.. https://doi.org/10.1073/pnas.90.18.8702

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