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Replication and physical parameters important for preparing purified Junin virus.

G Bushar·J L Sagripanti
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Abstract

Junin virus (JV) is an Arenavirus and the causative agent of Argentine hemorrhagic fever (AHF), an often fatal human disease. The attenuated strain XJ-clone 3 (XJC13) of JV, after being tested in humans, has been considered a promising vaccine. We found that synthesis of JV XJC13 reaches a peak 2 days after infection and the kinetics of synthesis are little affected by the multiplicity of infection (MOI) in a range from 0.125 to 1.00. Virus synthesis is sensitive to actinomycin D, indicating that cellular biosynthesis is required for viral replication. Combined precipitation and ultracentrifugation of supernatant from virus-infected cells yielded large amounts of concentrated and purified virion that banded in sucrose as a single peak with average density 1.177 +/- 0.015 g/ml. Purified virions have an average diameter of 203 +/- 23 nm by electron microscopy and an average sedimentation coefficient of 454 +/- 27 S. The results from the present study should assist in the preparation of large amounts of attenuated Junin virus which are required for vaccination against and diagnosis of Argentine hemorrhagic fever.

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AnimalsArenaviruses, New WorldDactinomycinKineticsMicroscopy, ElectronUltracentrifugationVero CellsVirus CultivationVirus ReplicationJournal Article

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G Bushar, J L Sagripanti. 1993. Replication and physical parameters important for preparing purified Junin virus.. https://doi.org/10.1016/0166-0934(93)90122-8

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