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PubMed · 8388843

Detection of human interferon-alpha-encoding gene expression.

E R Brandt·R J Devenish·B F Cheetham·A W Linnane
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Abstract

We have devised a sensitive method based on the polymerase chain reaction (PCR) to detect expression of human interferon-alpha-encoding genes (IFN-A) in general, and specifically, expression of the IFN-A2 or IFN-A4 genes. The utility of the PCR approach was assessed by analysis of cloned IFN-A genes, as well as genomic DNA and mRNA isolated from peripheral blood mononuclear cells. We demonstrate the specific amplification of sequences encoding IFN subtypes IFN-alpha-2 and IFN-alpha-4 from as little as 0.1 pg of IFN-A mRNA. The PCR technique has potential clinical application for the detection of IFN-A expression and, thus, identification of the IFN-alpha subtypes produced, particularly in small biopsy samples or otherwise, where only low numbers of cells are available.

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Base SequenceBlotting, SouthernChromosomes, Human, Pair 9Cloning, MolecularDNAGene ExpressionHumansInterferon-alphaMolecular Sequence DataMonocytesOligodeoxyribonucleotidesParainfluenza Virus 1, HumanPolymerase Chain ReactionRNA, MessengerTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov't

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E R Brandt, R J Devenish, B F Cheetham, A W Linnane. 1993-05-30. Detection of human interferon-alpha-encoding gene expression.. https://doi.org/10.1016/0378-1119(93)90730-q

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