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Purification, circular dichroism analysis, crystallization and preliminary X-ray diffraction analysis of the F plasmid CcdB killer protein.

J Steyaert·L Van Melderen·P Bernard·M H Thi·R Loris·L Wyns·M Couturier
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Abstract

Large crystals of the Escherichia coli F plasmid CcdB killer protein were grown from solutions containing 32% ammonium sulphate. The crystals belong to space group P4(2)2(1)2 with a = b = 104.52 A and c = 88.45 A or P2(1)2(1)2(1) with a = 77.62 A, b = 93.28 A and c = 141.44 A. Both crystal forms diffract to 2.6 A resolution. Structure determination by multiple isomorphous replacement is under way.

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Bacterial ProteinsBacterial ToxinsCircular DichroismCrystallizationEscherichia coliF FactorTopoisomerase I InhibitorsX-Ray DiffractionJournal ArticleResearch Support, Non-U.S. Gov't

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J Steyaert, L Van Melderen, P Bernard, M H Thi, R Loris, L Wyns, M Couturier. 1993-05-20. Purification, circular dichroism analysis, crystallization and preliminary X-ray diffraction analysis of the F plasmid CcdB killer protein.. https://doi.org/10.1006/jmbi.1993.1301

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